h PH phenotypic of CRI36 cotyledons infected with virus for 60 days.

g The mean PH of control check (CK) and pCLCrVA:GhPIN3 (VG) plants in Hai7124 cotyledons infected with virus for 30, 40, 50 and 60 days. f The mean expression level of GhPIN3 in control check (CK) and pCLCrVA:GhPIN3 (VG) plants of Hai7124, detected by qRT–PCR. e The mean PH of control check (CK) and pCLCrVA:GhPIN3 (VG) plants in CRI36 cotyledons infected with virus for 30, 40, 50 and 60 days. d The mean expression level of GhPIN3 in control check (CK) and pCLCrVA:GhPIN3 (VG) plants of CRI36, detected by qRT-PCR. c Expression patterns of GhPIN3 in mixture apical organs of eight varieties of BILs in the flowering period, detected by qRT–PCR red and blue dots indicate four tall-statured cotton and short-statured cotton, respectively. b Expression patterns of GhPIN3 in different tissues of CRI36 and Hai7124 varieties at three-leaf stage, detected by qRT-PCR. a Transcript profiles of promising genes for TM-1 ovule (O), fibre (F), root (R) and stem (S). Virus-induced gene silencing cotton has significantly increased PH when the expression of the GhPIN3 gene was suppressed.Įxpression level and function analysis of GhPIN3 in cotton. A qRT-PCR analysis showed that the expression level of GhPIN3 in apical tissues was significantly higher in four short-statured cotton genotypes than that in four tall-statured cotton genotypes. Based on functional annotation of orthologues in Arabidopsis and transcriptome data for the genes within the stable QTL regions, GhPIN3 encoding for the hormone auxin efflux carrier protein was identified as a candidate gene located in the stable QTL qPH-Dt1-1 region. In total, ten PH QTLs were identified and each explained 4.27–14.92% of the phenotypic variation, four of which were stable as they were mapped in at least two tests or based on best linear unbiased prediction in seven field tests. The high-density genetic map harboured 7,709 genotyping-by-sequencing (GBS)-based single nucleotide polymorphism (SNP) markers that covered 3,433.24 cM with a mean marker interval of 0.67 cM. barbadense L.) Hai7124 was used to construct a high-density genetic linkage map for quantitative trait locus (QTL) mapping. To facilitate the understanding of the genetic basis in PH, an interspecific backcross inbred line (BIL) population of 250 lines derived from upland cotton (Gossypium hirsutum L.) CRI36 and Egyptian cotton (G. Most published genetic studies on cotton have focused on fibre yield and quality traits rather than PH. Ideal plant height (PH) is important for improving lint yield and mechanized harvesting in cotton.

A novel gene GhPIN3 for plant height was identified in cotton. We constructed the first high-quality and high-density genetic linkage map for an interspecific BIL population in cotton by specific-locus amplified fragment sequencing for QTL mapping.

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